Wednesday, November 16, 2005

Cartilage Grown From Embryonic Stem Cells

Cartilage Grown From Embryonic Stem Cells

Researchers at Imperial College London have announced via press release that they have caused hESCc to successfully differentiate into cartilage tissue. The researchers grew them in culture along with cartilage cells; the mixed stem cells and cartilage were found to have higher levels of collagen, the protein constituent of cartilage, than stem cells cultured alone. The cells were then implanted in mice, and when the tissue scaffold (also see here) was removed 35 days later, the cells were found to have formed new cartilage. One of the researchers suggested that it may be as little as 5 years before treatment is possible.

Deriving Pluripotent Stem Cells from Sperm?

Deriving Pluripotent Stem Cells from Sperm?

In new research announced yesterday on EurekAlert, scientists at the University of Texas Southwestern have begun work attempting to derive embryonic-like stem cells from rat sperm stem cells. They have derived methods that keep the sperm stem cells from differentiating into sperm, and have also successfully frozen and then thawed and transplanted the sperm stem cells.

Besides being able to “push back” the stem cells into pluripotent stem cells, the researchers see the possibility of such things as male contraceptives and new ways to genetically modify laboratory animals. The scientists used a new medium to grow the cells in, and also used a genetically manipulated "tag" that specifically labeled germ cells with a green fluorescent protein.

A future step is to find out whether human male sperm stem cells can be “immortalized” in culture.

If pluripotent stem cells could be derived from sperm, would they face the same objections that stem cells derived from eggs do and be considered human life?

More on Korea

More on Korea

I’m getting kind of tired of reporting on politics instead of science, even though the two are linked in this field. Anyway….

Chosun Ilbo reports that the Korean ruling party is planning to fast-track legislation that would fund the Stem Cell Hub and make it a corporation separate from Seoul National University Hospital, with which it is currently affiliated. On the other hand, The Australian reported that Hugh Niall, chief executive officer of the Australian Stem Cell Center in Melbourne, and his colleagues would not meet with Hwang Woo-Suk next week at the AusBiotech conference in Perth.

At this point I think it’s a wait and see situation. With international support falling away and domestic support increasing, it seems that the World Stem Cell Hub may become the Korea Stem Cell Hub. I predict an increasing push for international regulation from Western governments. Asian nations such as India, Singapore, and Japan seem to have high stakes in stem cell research, and I would expect them to align with the West. I have no idea what will happen in Korea. Can the research go on without Hwang? Are the alleged ethical violations enough to ostracize all future research? Are we seeing a fundamental cultural divide? Does the South Korean ruling party represent the views of many Koreans? Stay tuned.

Another Heart Trial

Another Heart Trial

This news comes from press releases from the biotech companies involved, so there is not much scientific detail about the study. And obviously no results yet! But a clinical trial is starting this week in Mexico that will use a special bone marrow concentrate containing “all active forms of adult stem cells.” The stem cells will be injected into patients with chronic heart failure. This is a Phase I safety study. Given the results of other recent heart stem cell studies, I look for good news from this one.

The news is reported in Pacific Business News and Prime Zone.

Missouri Update

Missouri Update

The Missouri Secretary of State has allowed backers of stem cell research to begin circulating a petition to place an initiative on the ballot next year. This is reported (very) briefly in the Kansas City Star. The Kansas City Infozine gives details on the legislative processes, as well as the ballot language.

Missouri is presently a state divided about stem cell research, and I have posted on happenings there previously. The most recent newsworthy event (see Blog 11/9/05) was the decision of the Kansas-City based Stowers Institute to send money out of state because researchers do not want to come to Missouri, fearing that their work may become criminalized.

Ohio Moves Toward Banning hESC Research

Ohio Moves Toward Banning hESC Research

The Ohio Senate yesterday passed a bill that prohibits the state from funding embryonic stem cell research. Lines already approved by the federal government are exempt from the ban. According to Crain’s Cleveland Business, the bill passed mostly along party lines (21-11). The bill now moves to the Ohio House, where a similar bill was withdrawn because it did not appear to have the votes needed to pass. It criminalized human cloning.

The Toledo Blade quoted the Senate minority leader, Democrat C.J. Prentiss, as saying, “An investment that protects life is an investment that embraces research and allows us to protect that life.” Prentiss’s mother has Alzheimer’s Disease. The Blade also quoted Democrat Eric Fingerhut, who is Jewish, as saying,

The same God that created that embryo … created the miracle of its ability to differentiate and to create cures. That same God also created scientists who have vision and have the ability to see things that have never been seen. That's not some brain cell. That's a soul. That's a God-given talent.

The Akron Beacon Journal ran an AP wire story reporting that the bill bans human cloning but allows limited therapeutic cloning, such as to make skin for burn victims. However, according to the story, the language of the bill defines “embryo” in such a way as to actually prohibit even the limited therapeutic cloning. The story quoted an Ohio State University researcher, who said that the “ball of cells” that could be considered an embryo might never become viable. According to the story, 2/3 of the embryos of that stage that develop naturally also dies on their own.

The full text of the bill follows:

BE IT ENACTED BY THE GENERAL ASSEMBLY OF THE STATE OF OHIO:
Section 1. That section 131.51 of the Revised Code be enacted to read as follows:
Sec. 131.51. (A) As used in this section, "human cloning" means human asexual reproduction that is accomplished by introducing nuclear material from one or more human somatic cells into a fertilized or unfertilized oocyte whose nuclear material has been removed or inactivated so as to produce a living organism, at any stage of development, that is genetically virtually identical to an existing or previously existing human organism.
(B) Money in the state treasury and money in the custody of the treasurer of state shall not be used, directly or indirectly, to pay costs of or otherwise support any activities involving either of the following:
(1) Stem cell research with human embryonic tissue unless the stem cell research involves embryonic stem cells listed on the "Human Embryonic Stem Cell Registry" created by the national institutes of health in the United States department of health and human services in accordance with presidential criteria established on August 9, 2001, or unless the stem cell research involves embryonic stem cells derived in a manner that does not destroy the embryo;
(2) Human cloning.
(C) Nothing in division (B)(2) of this section shall restrict the use of such money with regard to areas of scientific research that do not involve the creation or use of an embryo produced by human cloning or any product derived from an embryo produced by human cloning. Areas of research with regard to which the use of such money is not restricted by that division include, but are not limited to, the use of nuclear transfer or other cloning techniques to produce molecules, human deoxyribonucleic acid, tissues, organs, plants, animals other than humans, and cells other than human embryos.

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The debate seems the same here as it has been in other states, and we will just have to see what happens.

Oversight Issues

Oversight Issues

Reuters ran an interesting article yesterday in which scientists discussed the issues of oversight for stem cell research and obtaining eggs. Scientists said that extremely careful regulation was needed because the technology was still so new and suggested international guidelines and regulation might be necessary. One researcher said that countries which forbid embryonic stem cell research should export spare embryos. She also thought it was a double standard for those countries to later benefit from the therapies.

The article does not bring up many new points for people who have been following the stem cell debates, but it is a succinct explanation of the issues.